Flexibility for Multicolor Panel Design
Optimizing immunophenotyping panels is a time consuming, iterative process. A number of markers are required to identify the populations of interest and then additional markers to interrogate function. Finding reagents that have the markers conjugated to fluorochromes detectable on the instrumentation available is another challenge. Ensuring that the reagent combination doesn’t compromise sensitivity often requires multiple rounds of empirical testing. A typical 12 color panel can require 1-4 months of design and optimization (1). The addition of a viability dye to the panel can prove crucial to obtaining the results needed. Having the flexibility to add a fixable viability dye in a variety of channels makes it easier to incorporate these into the design.
| Part Number | Reagent | Excitation/Emission (nm) | Excitation Laser | Bandpass |
|---|---|---|---|---|
| C36614 | ViaKrome 405 Fixable Viability Dye | 401/420 | Violet 405 nm | 450/45 |
| C36620 | ViaKrome 561 Fixable Viability Dye | 555/565 | Blue 488 nm | 585/42 |
| C36620 | ViaKrome 561 Fixable Viability Dye | 555/565 | Yellow Green 561 nm | 585/42 |
| C36624 | ViaKrome 638 Fixable Viability Dye | 638/655 | Red 638 nm | 660/10 |
| C36628 | ViaKrome 808 Fixable Viability Dye | 854/878 | Infrared 808 nm | 885/40 |
1. Mahnke, Y. , Chattopadhyay, P. and Roederer, M. (2010), Publication of optimized multicolor immunofluorescence panels. Cytometry, 77A: 814-818. doi:10.1002/cyto.a.20916