RNA- und cDNA-Aufbereitung

RNAClean XP

Reinigung der RNA und cDNA von häufigen enzymatischen Reaktionen unter Verwendung unserer geschützten paramagnetischen Bead-basierten SPRI-Chemie.

  • Kompatibel mit manueller und automatischer Verarbeitung
  • Vollständige Entfernung von Salzen, nicht aufgenommener Primer und dNTPs
  • Wird in RNA-Seq-Bibliothekszubereitungen verwendet
  • Zertifiziert RNAse-frei

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Resources + Tools
Performance Resources Technical Documents Training Liquid Handlers Support

Explore RNAClean XP Reagents

Explore RNAClean XP Reagents

RNAClean XP Workflow Steps

Genomics RNAClean XP Workflow

Product Features

Overview

Das Agencourt RNAclean XP - Kit bietet mittels der einzigartigen Solid Phase Reversible Immobilization (SPRI) - Technologie, basierend auf magnetischen Beads, eine einfache, flexibele sowie hervorragend reproduzierbare Aufreinigung von enzymatischen Reaktionen wie der cDNA-Synthese und der in-vitro-Transkription (IVT).

Eigenschaften
•Aufreinigung kleiner und größerer Templates
•Vollständiges Entfernen von Salzen, Primern sowie dNTPs
•Einfaches, leicht zu automatisierendes Protokoll
•Keine Zentrifugation oder Vakuumfiltration
•Einfache Elution in wässrigen Lösungen

High Recovery

Efficient recovery of your sample ensures that critical data is not lost within the workflow.

Flexible

Manual, hands-on cleanups using RNAClean XP require less than 30 minutes.

Scalable

For higher throughput needs, RNAClean XP is automated on Biomek platforms enabling hundreds of samples per day.

Citations

Donega, V., Burm, S. M., van Strien, M. E., van Bodegraven, E. J., Paliukhovich, I., Geut, H., . . . Hol, E. M. (2019). Transcriptome and proteome profiling of neural stem cells from the human subventricular zone in Parkinson’s disease. Acta Neuropathologica Communications, 7(4). doi:10.1186/s40478-019-0736-0

  • Used to clean up amplified RNA

Warner, A. D., Gevirtzman, L., Hillier, L. W., Ewing, B., & Waterston, R. H. (2019, May 23). The C. elegans embryonic transcriptome with tissue, time, and alternative splicing resolution. Genome Research. doi:10.1101/gr.243394.118

  • Used to clean up RNA post rRNA removal

Caro-Vegas, C., Bailey, A., Bigi, R., Bamania, B., & Dittmer, D. P. (2019, January/February). Targeting mTOR with MLN0128 Overcomes Rapamycin and Chemoresistant Primary Effusion Lymphoma. mBio, 10(1), e02871-18. doi:10.1128/mBio.02871-18

  • Used to clean up fragmented RNA and cDNA 

Disclaimer: Beckman Coulter makes no warranties of any kind whatsoever express or implied, with respect to this protocol, including but not limited to warranties of fitness for a particular purpose or merchantability or that the protocol is non-infringing. All warranties are expressly disclaimed. Your use of the method is solely at your own risk, without recourse to Beckman Coulter. Not intended or validated for use in the diagnosis of disease or other conditions. This protocol is for demonstration only, and is not validated by Beckman Coulter.

Content and Resources

Flyer: Genomic Solutions Collaboration Program (PDF) Through this program, you’ll have exclusive access to Beckman Coulter Life Sciences genomic technologies, data analysis tools and application scientists.
Brochure - Genomic Reagent Solutions Next-Generation Sequencing (NGS) Cleanup & Size Selection
Genomic Reagent Solutions for NGS Cleanup and Size Selection – Brochure for OEM Partners Explore portfolio and capabilities of our genomic reagents for NGS cleanup and size selection and learn about how you can streamline your NGS workflows
SPRI Methodology Solid Phase Reversible Immobilization (SPRI) technology, uses paramagnetic beads to selectively bind nucleic acids by type and size.
OEM Reagent Kits Flyer: By partnering with us as an OEM supplier to develop your genomic assays, you’re committing to a solution that you and your customers can rely on.

Technische Dokumente

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