Entfernung des Sanger Farbstoff-Terminators

CleanSEQ-Bereinigung und Größenauswahl

Ein schnelles, leistungsstarkes Verfahren zur Entfernung von Farbstoff-Terminatoren unter Verwendung einer paramagnetischen Bead-basierten Technologie.

  • Erfordert keine Zentrifugation oder Filtration
  • Wird manuell oder vollautomatisch durchgeführt, um den Farbstoff-Terminator mit hohem Probendurchsatz zu entfernen
  • Erzeugt Sequenzen mit längeren Phred 20-Messwertlängen und höheren Signalintensitäten als andere Aufreinigungstechnologien

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CleanSEQ Sanger Sequencing Dye Terminator

Explore CleanSEQ Reagents

Explore CleanSEQ Reagents

Product Features

Overview

CleanSEQ is a highly efficient, easily automated sequencing purification system that removes Big Dye terminator and sequencing reaction contaminants.

Scalable

Compatible with common sequencing chemistries and platforms in both 96- and 384-well formats. CleanSEQ is automation-friendly allowing both high throughput and format flexibility.

Consistently Delivers

Long Phred20 read lengths averaging over 700 bps and pass rates of 85% or higher. Increased average signal strength - efficient elimination of sequecing reaction contaminants.

High Quality Sequencing Results

Higher average signal intensity compared to ethanol precipitation, gel filtration, and silica-based magnetic reagents.

Content and Resources

Reproducible and Efficient Automation of DTR Purification Using the Biomek 4000 Workstation and CleanSEQ The Agencourt CleanSEQ application for the Biomek 4000 Laboratory Automation Workstation is a rapid, high performance dye-terminator removal method utilizing Solid Phase Reversible Immobilization (SPRI) paramagnetic beadbased technology.
Agencourt CleanSEQ System Dye Terminator Removal Scalable, automation friendly and high throughput PCR purification.
Automated Demonstrated Methods for Biomek i-Series Workstations Learn more about automated NGS Library Construction and Nucleic Acid Sample Preparation methods for Biomek i-Series workstations
SPRI Methodology Learn more about SPRI technology which uses paramagnetic beads to selectively bind nucleic acids by type and size.
OEM Reagent Kits Flyer: By partnering with us as an OEM supplier to develop your genomic assays, you’re committing to a solution that you and your customers can rely on.

Technische Dokumente

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