DNA-Isolierung aus FFPE-Gewebe

In zunehmenden Maße sind formalinfixierte und paraffin-eingebettete Gewebeproben (FFPE-Proben) ein wesentlicher Bestandteil vieler Next-Generation-Sequenzierung (NGS) Probenvorbereitungs-Workflows, speziell im Bereich der Krebsforschung. Hochqualitative Extraktionsprotokolle, die hohe Nukleinsäureausbeuten und -reinheiten ermöglichen sind essenziell um verlässliche Forschungsergebnisse zu erzielen. FormaPure DNA bietet jetzt ein automatisiertes, SPRI-basiertes Extraktionsreagenz-Kit, um Ihre sich entwickelnden Forschungsbedürfnisse zu unterstützen. Vor allem bietet es erhebliche Zeiteinsparungen in Form von reduzierten Laufzeiten und weniger als 15 Minuten Hands-on-Zeit, wenn das Kit automatisiert auf einem Biomek Liquid Handler verwendet wird.

  • Minimale Hands-on Zeit
  • Gesteigerte Produktivität durch Automation - niedrieger bis hoher Probendurchsatz
  • Bewährte Kompatibilität mit NGS und anderen Downstream-Anwendungen
  • Zuverlässige Extraktionsleistung
  • Erhöhte Laborsicherheit

Explore Aus FFPE-Gewebe Models

FormaPure XL DNA Visual Workflow

Genomics FormaPure XL DNA Workflow

FormaPure XL DNA Features

Overview
FormaPure XL DNA isolates DNA from a variety of formalin-fixed, paraffin-embedded (FFPE) samples and tissue types.

Simplified Workflow
FormaPure XL DNA offers a user friendly manual workflow with minimal hands-on time and no volatile chemicals.

Product Specifications: FormaPure XL DNA

Application Uses DNA Extraction, DNA Isolation, DNA sequencing, Nucleic Acid Sample Prep
Format Liquid
Starting Sample Material FFPE (formalin fixed paraffin embedded) tissue.
Automated Available Yes
Item Specifications Referenced C35996

Content and Resources

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Resource Type
Automating Genomic DNA Extraction from Whole Blood and Serum with GenFind V3 on the Biomek i7 Hybrid Genomic Workstation Here, we demonstrate a walk-away automated solution for GenFind V3 kit to purify up to 400 µL of whole blood using the Beckman Coulter Biomek i7 Hybrid Genomics Workstation.
Automated library preparation for the MCI Advantage Cancer Panel at Miami Cancer Institute utilizing the Beckman Coulter Biomek i5 Span-8 NGS Workstation Here we describe the automation of the MCI Advantage Cancer Panel at Miami Cancer Institute, a 170 gene panel assay based upon the Illumina TruSight® Tumor 170 Panel, on the Beckman Coulter Biomek i5 Span-8 NGS Workstation
Isolation and Separation of DNA and RNA from a Single Tissue or Cell Culture Sample We are currently developing a product using proprietary bead based technology that selectively binds DNA to the beads to effectively separate DNA and RNA independently. This allows for the extraction of all nucleic acids from one experimental setup
A complete workflow for high-throughput isolation of DNA and RNA from FFPE samples using Formapure XL Total on the KingFisherâ„¢ Sample Purification System This application note demonstrates the use of FormaPure XL Total, a total nucleic acid extraction kit for FFPE samples, in conjunction with the KingFisherâ„¢ Duo Prime Sample Purification System as a potential solution that mitigates some of the challenges with FFPE workflows.

Technische Dokumente

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