Genomik Reagenzien Auswahlhilfe

Beckman Coulters Genomik Reagenzien nutzen die Solid Phase Reversible Immobilization (SPRI) –Technologie, die weithin bekannt für den Einsatz in unserem Agencourt AMPure XP-Kit ist. Dabei werden paramagnetische Partikel verwendet um Nukleinsäuren selektiv nach Art und Größe zu binden. SPRI ermöglicht hochleistungsfähige Isolierungs- und Aufreinigungsprotokolle für Anwendungen wie qPCR, ddPCR, Sanger-Sequenzierung, Next-Generation-Sequencing (NGS) und Microarrays. 

Sehen Sie sich die unten stehende Liste an um nach den Probenmaterialien und Nukleinsäuretypen zu suchen, die Sie für Ihre manuelle oder automatisierte Probenvorbereitung verwenden. Bitte beachten Sie, dass die unten aufgeführten Probenmaterialien lediglich einen Überblick über die verfügbaren Kits darstellen und nicht ausschließlich auf diese Liste beschränkt sind.

 

STARTING MATERIAL DNA ISOLATION RNA ISOLATION PURIFICATION & CLEAN-UP
CosMC Prep
DNAdvance
FormaPure DNA1
GenFind v2
FormaPure
RNAdvance Blood
RNAdvance Cell v2
RNAdvance Tissue
AMPure XP
CleanSeq
RNAClean XP
SPRIselect
MAMMALIAN SAMPLES
Buccal Swabs
 
FFPE Tissue
 
 
FTA Swabs
 
Oragene Saliva Collection Tubes
 
Plasma
 
Serum
 
Tissue
 
 
Whole Blood
 
 
CELL CULTURES
293 T
 
 
Adherent
 
 
CHO
 
 
HeLa
 
 
Hepatocytes <50k Cells
 
 
Hepatocytes ≥50k Cells
 
 
Non-Adherent
 
 
BACTERIA
B. subtilis (Gram +)
 
 
 
E. coli (Gram –)
 
 
 
PLASMIDS
BACs, Cosmids, Fosmids
 
OTHER MATERIALS
Avian Blood
 
Insects (including Drosophila)
 
 
 
Zebra Fish
 
 
NUCLEIC ACID SAMPLES
cDNA Synthesis
 
 
Dye Terminator Removal
 
 
 
In vitro Transcription
 
 
NGS Library Construction Steps
 
 
 
PCR Products
 

Highlighted Extraction Protocols

Sample Types from Customers and Protocols Developed

Sample Type Title of Supplemental Protocol or Kit Suggested
PAXgene Blood DNA collection tube Whole Blood DNA extraction from PAXgene Blood DNA Tube using GenFind V3
FTA/blood spots DNA extraction from FTA cards or filter paper blood spots using GenFind V3
Large volume blood DNA extraction from up to 2mL of Whole Blood
PBMCs DNA extraction from a large cell number 
Archaeological bone samples Contact PoP Team for help with this sample type
Formalin Fixed Autopsy Samples
Formalin-fixed and non-paraffin-embedded tissue DNA extraction
Feces
Contact PoP Team for help with this sample type
Liquid Biopsy
Apostle MiniMax
Mouthwash
DNA extraction from Mouthwash using GenFind V3
Zeesan Saliva
DNA extraction from Zeesan Saliva DNA sample collection kit using GenFind V3
Swabs
Isolation of DNA from Buccal Swabs using DNAdvance
Urine Pellets
Contact PoP Team for help with this sample type
Fibrotic Tissue
Contact PoP Team for help with this sample type
Fatty Tissue
Contact PoP Team for help with this sample type
Fresh/frozen tissue
DNAdvance
FFPE tissue
FormaPure XL
Fixed Tissue
Formalin-fixed and non-paraffin-embedded tissue DNA extraction
Bacteria (E. coli, Bacillus and others) DNA
DNA Isolation from Bacteria using GenFind V3
Bacteria (E. coli, Bacillus and others) RNA
RNA Isolation from Bacteria using RNAdvance Tissue
Bacteria (mucoid P. seudomonas) DNA
Extraction of DNA from Mucoid Bacteria using GenFind V3
Bacteria (mucoid P. seudomonas) RNA
RNA Isolation from Mucoid Bacteria with RNAdvance Tissue Kit
Bacteria in Urine
Contact PoP Team for help with this sample type
Viral RNA or DNA
Viral Nucleic Acid extraction using RNAdvance Blood
Fungi
DNA extraction from Filamentous Fugni using GenFind V3
Fungus from fingernail clippings
Contact PoP Team for help with this sample type
Yeast
Contact PoP Team for help with this sample type
HEK 293 T Cells, TC cells, C2C12 Cell line
DNA extraction from cell culture using GenFind V3
High Molecular Weight (HMW) DNA extraction
Contact PoP Team for help with this sample type
DNA and RNA extraction from one sample
Simultaneous DNA & RNA Extraction from Tissue without Splitting the Lysate
Plants (tobacco)
Contact PoP Team for help with this sample type

 

Beckman Coulter makes no warranties of any kind whatsoever express or implied, with respect to this protocol, including but not limited to warranties of fitness for a particular purpose or merchantability or that the protocol is non-infringing. All warranties are expressly disclaimed. Your use of the method is solely at your own risk, without recourse to Beckman Coulter. Not intended or validated for use in the diagnosis of disease or other conditions. This protocol is for demonstration only, and is not validated by Beckman Coulter.
© 2019 Beckman Coulter, Inc. All rights reserved. Beckman Coulter, the stylized logo, and the Beckman Coulter product and service marks mentioned herein are trademarks or registered trademarks of Beckman Coulter, Inc. in the United States and other countries.

Beckman Coulter Life Sciences partners to offer Apostle MiniMax™ products, a trademark of Apostle, a liquid biopsy company.

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