Extraktion von Zellen
Das Agencourt RNAdvance Cell v2-Kit basiert auf der paramagnetischen Bead-Technologie Solid Phase Reversible Immobilization (SPRI), und extrahiert reine Nukleinsäuren höchster Qualität in hohen Ausbeute. Das System ermöglicht zuverlässig eine hohe RNA-Ausbeute in hoher Reinheit ohne dabei zusätzliche Filtrations oder Zentrifugationsschritte zu benötigen. Bei der Agencourt RNAdvance Cell v2-Chemie handelt es sich um einen automatisierungsfreundlichen Prozess zur Isolierung der Gesamt-RNA zur Nutzung bei Microarray- und Echtzeit-qPCR-Anwendungen.
Explore Aus Zellen Models
RNAdvance Cells Workflow Steps
Product Features
RNAdvance Cell V2 isolates total RNA from cultured cells.
The RNAdvance Cell V2 isolation kit purifies RNA with high RIN scores enhancing suitability for downstream applications.
Combining RNAdvance Blood chemistry with our Biomek Workstation offers high throughput and minimal hands-on solutions: two 96-well plates of samples can be purified in approximately 2 hours and 15 minutes.
Product Specifications
| Application Uses | RNA Isolation, RNA Extraction, RNA Sequencing, Nucleic Acid Sample Prep |
| Format | Liquid |
| Starting Sample Material | Cultured Cells |
| Automated Available | Yes |
| Item Specifications Referenced | A47943 |
Citations
Hore, T. A., von Meyenn, F., Ravichandran, M., Bachman, M., Ficz, G., Oxley, D., . . . Reik, W. (2016, October 25). Retinol and ascorbate drive erasure of epigenetic memory and enhance reprogramming to naïve pluripotency by complementary mechanisms. PNAS, 113(43), 12202-1207. doi:10.1073/pnas.1608679113
- Used to extract RNA from ESC and EpiSC cell lines
Mesnage, R., Phedonos, A., Biserni, M., Arno, M., Balu, S., Corton, J. C., . . . Antoniou, M. N. (2017, October). Evaluation of estrogen receptor alpha activation by glyphosate-based herbicide constituents. Food and Chemical Toxicology, 108, 30-42. doi:10.1016/j.fct.2017.07.025
- Used to extract RNA from MCF-7 cells
Disclaimer: Beckman Coulter makes no warranties of any kind whatsoever express or implied, with respect to this protocol, including but not limited to warranties of fitness for a particular purpose or merchantability or that the protocol is non-infringing. All warranties are expressly disclaimed. Your use of the method is solely at your own risk, without recourse to Beckman Coulter. Not intended or validated for use in the diagnosis of disease or other conditions. This protocol is for demonstration only, and is not validated by Beckman Coulter.