Extraktion von Zellen

Das Agencourt RNAdvance Cell v2-Kit basiert auf der paramagnetischen Bead-Technologie Solid Phase Reversible Immobilization (SPRI), und extrahiert reine Nukleinsäuren höchster Qualität in hohen Ausbeute. Das System ermöglicht zuverlässig eine hohe RNA-Ausbeute in hoher Reinheit ohne dabei zusätzliche Filtrations oder Zentrifugationsschritte zu benötigen. Bei der Agencourt RNAdvance Cell v2-Chemie handelt es sich um einen automatisierungsfreundlichen Prozess zur Isolierung der Gesamt-RNA zur Nutzung bei Microarray- und Echtzeit-qPCR-Anwendungen.

Resources + Tools
Performance Technical Documents Training Liquid Handlers Support FAQs

Explore Aus Zellen Models

RNAdvance Cells Workflow Steps

Genomics RNAdvance Cell v2 Workflow

Product Features

Overview
RNAdvance Cell V2 isolates total RNA from cultured cells.

High Quality RNA
The RNAdvance Cell V2 isolation kit purifies RNA with high RIN scores enhancing suitability for downstream applications.

Flexible and Scalable
Combining RNAdvance Blood chemistry with our Biomek Workstation offers high throughput and minimal hands-on solutions: two 96-well plates of samples can be purified in approximately 2 hours and 15 minutes.

Product Specifications

Application Uses RNA Isolation, RNA Extraction, RNA Sequencing, Nucleic Acid Sample Prep
Format Liquid
Starting Sample Material Cultured Cells
Automated Available Yes
Item Specifications Referenced A47943

Citations

Hore, T. A., von Meyenn, F., Ravichandran, M., Bachman, M., Ficz, G., Oxley, D., . . . Reik, W. (2016, October 25). Retinol and ascorbate drive erasure of epigenetic memory and enhance reprogramming to naïve pluripotency by complementary mechanisms. PNAS, 113(43), 12202-1207. doi:10.1073/pnas.1608679113

  • Used to extract RNA from ESC and EpiSC cell lines

Mesnage, R., Phedonos, A., Biserni, M., Arno, M., Balu, S., Corton, J. C., . . . Antoniou, M. N. (2017, October). Evaluation of estrogen receptor alpha activation by glyphosate-based herbicide constituents. Food and Chemical Toxicology, 108, 30-42. doi:10.1016/j.fct.2017.07.025

  • Used to extract RNA from MCF-7 cells

Disclaimer: Beckman Coulter makes no warranties of any kind whatsoever express or implied, with respect to this protocol, including but not limited to warranties of fitness for a particular purpose or merchantability or that the protocol is non-infringing. All warranties are expressly disclaimed. Your use of the method is solely at your own risk, without recourse to Beckman Coulter. Not intended or validated for use in the diagnosis of disease or other conditions. This protocol is for demonstration only, and is not validated by Beckman Coulter.

Content and Resources

Isolation of RNA from Bacteria using RNAdvance Tissue Kit This protocol optimizes RNA isolation from a wide range of bacteria using RNAdvance Tissues with lysozyme
Simultaneous DNA & RNA Extraction from Cultured Cells without Splitting Lysate We developed a protocol using Beckman Coulter reagents to isolate both DNA and RNA from the same cultured cell sample. The method utilizes a proprietary buffer to selectively bind RNA and DNA
FormaPure Total: Protocol for DNA and RNA Isolation from the Same FFPE Sample This document outlines the instructions for using the FormaPure Total extraction and purification kit.
FormaPure Total Quick Guide Checklist instructions for using the FormaPure Total extraction and purification kit.
FormaPure RNA: Extended Protocol for RNA Isolation from FFPE Sample This document provides instructions for using the FormaPure RNA extraction and purification kit.

Technische Dokumente

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