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What variables can affect accuracy of sedimentation and diffusion coefficient calculations?
Location between the two menisci (i.e., it must be properly selected)
Density of the solvent
Temperature (affects viscosity)
Concentration dependency (pure, non-associating solutes show a decrease in sedimentation coefficient with an increase in concentration)
Radial dilution due to sector shapes
Boundary analysis (concentration and shape)
Speed dependence (sedimentation coefficient may increase with speed increase, and result in solute aggregation)
Primary electrostatic charge of solute and solvent
Association reactions that might occur between molecules as molecular weight increases
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