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Krome Orange dye conjugates can be used on any flow cytometer equipped with violet excitation and appropriate emission filters. Data presented using Krome Orange conjugates of anti-human CD4, CD19 and CD45 in multicolor applications, including side scatter/CD45 gating and a 6-plus color stain. Krome Orange dye provides optimized second organic fluorochrome for violet excitation, enabling 10-color applications on the Gallios / Navios flow cytometer systems.