AMPure XP PCR-Aufreinigungssystem

Das Agencourt AMPure XP System ist ein hocheffizientes, automationsfreundliches PCR-Aufreinigungssystem, das hohe Qualität ohne Salzverschleppung liefert. Es benötigt keine Zentrifugation oder Filtrationsschritte, so kann Agencourt AMPure XP problemlos in manuellen und automatisierten 96- oder 384-Well-Formaten eingesetzt werden.

AMPure XP Workflow

AMPure XP Workflow

Product Features

Agencourt AMPure XP Eigenschaften

• Hohe Rückgewinnung von Amplicons, größer als 100 bp
• Effiziente Entfernung von uninkorporierten dNTPs, Primern, Primer-Dimeren, Salzen und anderen Verunreinigungen
• Stabile PCR-Produkte nach der Aufreinigung: Kein PCR-Abbau nach Lagerung bei 4 ° C für sieben Tage
• Effiziente Wiedergewinnung von doppelsträngigen und einzelsträngigen DNA-Schablonen
• Schnelle manuelle und automatisierte PCR-Aufreinigug

Nachfolgende Anwendungstechniken

PCR, Genotypisierung (SNP-Detektion) Fragmentanalyse, Sequenzierung (Sanger & Next Generation), Klonen, Primer Walking

Agencourt Prozessübersicht

1. PCR-Reaktion 2. Bindung von PCR-Amplicons an magnetische Beads 3. Trennung von PCR-Amplicons, die an magnetische Beads und Verunreinigungen gebunden sind 4. Waschen von PCR-Amplicons mit Ethanol 5. Elution von PCR-Amplicons von den magnetischen Partikeln 6. Transfer der Beads in eine neue Platte

Gold Standard for NGS Workflows
Trusted and recommended reagent for size selection and cleanup steps within a variety of NGS workflows.

Flexible and Scalable
Manual cleanups using AMPure XP require less than 30 minutes. For higher throughput needs, AMPure XP is automated on all Biomek platforms enabling hundreds of samples per day.

Agencourt AMPure XP

Part Number Product Name Volume Format Listenpreis Kaufen

1 x 5 mL

Liquid

€326.50

60 x mL

Liquid

€781.60

450 x mL

Liquid

€4,442.00

Product Specifications

Application Uses Purification & Clean-up, PCR Purification, DNA Purification, Sequencing, NGS Clean-up, PCR clean-up
Format Liquid
Starting Sample Material DNA
Automated Available Yes
Item Specifications Referenced A63882

Don't Lose Critical Data

When so much more has been invested in your research, AMPure XP is the only choice for purification and clean-up steps. Loss of yield during this critical step leads to loss of discovery in your research.

Relative costs of the different steps required to perform various NGS applications. Steps include Extraction, Library Construction, Library Enrichment, Clean-up, and Sequencing. Costs were calculated based on average list price of commercially available kits and reagents in 2017. Clean-up efficiencies were calculated by determining the total DNA yield by Picogreen Assay after performing a clean-up procedure on a known amount of DNA. The percent yield relative to AMPure XP performance was then used to calculate the impact of efficiency on various commercially available library construction methods and a change in purification reagent.

AMPure XP - Keep Critical Data For Downstream Applications

Brochures & Other Materials

Document Title Category Language
Documentation
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RNA Isolation From FFPE Tissue
Application Notes
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RNA Isolation From FFPE Tissue
Documentation
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FFPE Tissue
Documentation
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LUCID Custom Panel Design & Cocktail Services
English
Documentation
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LUCID Custom Panel Design & Cocktail Services, RESOURCE Contract Manufacturing Services
English
Documentation
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LUCID Custom Panel Design & Cocktail Services, RESOURCE Contract Manufacturing Services
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